PerCP/Cyanine5.5 anti-human MICA/MICB Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2112	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemistry^2,3,5 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation⁷, and blocking^2,3 of MIC mediated cytotoxicity. The Ultra-LEAF? Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 320919 & 320120).

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Groh V, et al. 1999. Science 279:1737.
Groh V, et al. 1999. Proc. Natl. Acad. Sci. USA. 96:6879.
Groh V, et al. 2001. Nature Immunol. 2:255.
Li Z, et al. 2000. Immunogenetics 51:246.
Park EJ, et al. 2003. J. Immunol. 171:4131.
Jinushi M, et al. 2003. J. Immunol. 171:5423.
Wu J, et al. 2003. J. Immunol. 170:4196.

RRID

AB_2715908 (BioLegend Cat. No. 320915) AB_2715909 (BioLegend Cat. No. 320916)

Antigen Details

Structure: 65-75 kD glycoprotein, MHC class I-like transmembrane molecules that do not associate ?2-microglobulin, highly polymorphic
Distribution: Intestinal epithelium, epithelial tumors, endothelial cells, fibroblasts, INF-a-stimulated dendritic cells
Function: Activation of NK cells, subset of CD8⁺ α/? T cells and γ/δ T cells, suppression of T cell proliferation
Ligand/Receptor: NKG2D
Cell Type: Dendritic cells, Endothelial cells, Epithelial cells, Fibroblasts
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Groh V, et al. 1996. Proc. Natl. Acad. Sci. USA. 93:12445.
2. Groh V, et al. 1999. Proc. Natl. Acad. Sci. USA. 96:6879.
3. Jinushi M, et al. 2003. J. Immunol. 170:1249.
4. Kriegeskorte AK, et al. 2005. Proc. Natl. Acad. Sci. USA. 102:11805.
5. Groh V, et al. 1999. Science 279:1737.
Gene ID: 100507436 View all products for this Gene ID 4277 View all products for this Gene ID
UniProt: View information about MICA MICB on UniProt.org

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