Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), ELISA,?immunoprecipitation (IP), and flow cytometry (FC).

6D11 reacts with both the PrPc and PrPsc forms. The epitope falls within amino acids 93-109 of PrP.

1. Kondoh G, et al. Angiotensin-converting enzyme is a GPI-anchoredprotein releasing factor crucial for fertilization. Nat Med 11(2):160-66, 2005. (IHC)
2. Gilch S, et al. Recognition of lumenal prion proetin aggregates bypost-ER quality control mechanisms is mediated by the preoctarepeat region of PrP. Traffic 5:300-313, 2004. (WB, IHC, ELISA)
3. Nishina K, et al. In vitro prion protein conversion in detergentsolubilized membranes. Biochem 43:2613-2621, 2004. (IP)
4. Shiga Y, et al. Diffusion-weighted MRI abnormalities as an early marker for Creutzfeldt-Jakob disease. Neurol 63:443-449, 2004. (WB, IHC, IP, ELISA)
5. Zou W, et al. Antibody to DNA detects scrapie but not normal prion protein. PNAS 101(5):1380-1385, 2004.
6. Kitamoto T, et al. Formic acid pretreatment enhances immunostaining of cerebral and systemic amyloids. Lab Invest 57:230-236, 1987.
7. Kascsak, et al. Mouse polyclonal and monoclonal antibody to scrapie associated fibril proteins. J Virol 61:3688-3693, 1987.
8. Spinner DS, et al. 2007. J. Leuko. Biol. 81:1374. (IHC, WB)

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