Alexa Fluor® 647 anti-CD230 (Prion) Antibody
- 产品名称：
- Alexa Fluor® 647 anti-CD230 (Prion) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 808007
- 产品应用：
- 808007
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), ELISA,?immunoprecipitation (IP), and flow cytometry (FC).
6D11 reacts with both the PrPc and PrPsc forms. The epitope falls within amino acids 93-109 of PrP.
- Application References
(PubMed link indicates BioLegend citation) -
- Kondoh G, et al. Angiotensin-converting enzyme is a GPI-anchoredprotein releasing factor crucial for fertilization. Nat Med 11(2):160-66, 2005. (IHC)
- Gilch S, et al. Recognition of lumenal prion proetin aggregates bypost-ER quality control mechanisms is mediated by the preoctarepeat region of PrP. Traffic 5:300-313, 2004. (WB, IHC, ELISA)
- Nishina K, et al. In vitro prion protein conversion in detergentsolubilized membranes. Biochem 43:2613-2621, 2004. (IP)
- Shiga Y, et al. Diffusion-weighted MRI abnormalities as an early marker for Creutzfeldt-Jakob disease. Neurol 63:443-449, 2004. (WB, IHC, IP, ELISA)
- Zou W, et al. Antibody to DNA detects scrapie but not normal prion protein. PNAS 101(5):1380-1385, 2004.
- Kitamoto T, et al. Formic acid pretreatment enhances immunostaining of cerebral and systemic amyloids. Lab Invest 57:230-236, 1987.
- Kascsak, et al. Mouse polyclonal and monoclonal antibody to scrapie associated fibril proteins. J Virol 61:3688-3693, 1987.
- Spinner DS, et al. 2007. J. Leuko. Biol. 81:1374. (IHC, WB)
- RRID
- AB_2783384 (BioLegend Cat. No. 808007) AB_2783385 (BioLegend Cat. No. 808008)
