Purified anti-complement C3/C3b/iC3b Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3180	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human C3

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

WB - Quality tested
FC, Direct ELISA - Verified
ELISA Detection - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 ?g per ml.?For flow cytometric staining, the suggested use of this reagent is 1.0 - 2.0 ?g per million cells in 100 ?l volume. For Direct ELISA, the suggested use of this reagent is 0.01 - 2.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes human C3, C3b and iC3b, and does not cross-react with C3d.

Personal communication from the developer of clone 6C9/C3b indicates it recognizes mouse and non-human primate C3, C3b, and iC3b.

Application References (PubMed link indicates BioLegend citation)

Shi J, et al. 2014. Blood. 123:4015. (FC)

RRID

AB_2572183 (BioLegend Cat. No. 846502)

Antigen Details

Structure: C3 is a 185 kD glycoprotein that is cleaved to generate a 176 kD protein fragment, C3b. iC3b is proteolytically derived from C3b and exists as a mixture of 176 and 174 kD proteins.
Distribution: Extracellular, plasma membrane, and serum.
Function: C3b is involved in all three complement pathways and is essential for effective complement activation and presentation of antigens to cells of the adaptive immune system. iC3b is less active than C3b, but the target-bound protein can expand target-specific B-cell and T-cell populations by interacting with lymphoid and phagocytic cells.
Interaction: Immune cells interact with C3b and iC3b using CR1 and CR2/CR3, respectively.
Ligand/Receptor: C3b can bind to Factor B, Factor P, Factor H, Factor I, C5, DAF (CD55), MCP (CD46), and CR1 (CD35). iC3b interacts with CR2 (CD21) and CR3 (CD11b/CD18).
Biology Area: Cell Biology, Complement, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References: 1. Lambris JD, et al. 1988. Immunol. Today 9:387-93.
Gene ID: 718 View all products for this Gene ID
UniProt: View information about C3/C3b/iC3b on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们