PE anti-mouse Ly-51 Antibody
- 产品名称：
- PE anti-mouse Ly-51 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 108307
- 产品应用：
- 108307
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- C57BL/6 mouse Pre-B lymphoma cell line (L1-2) plus Abelson murine leukemia virus-specific cytotoxic T-cell clones
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2 and immunohistochemical staining3-5, 7-8 of acetone-fixed frozen sections.
- Application References
(PubMed link indicates BioLegend citation) -
- Pillemer E, et al. 1984. P. Natl. Acad. Sci. USA 81:4434. (IP)
- Hardy RR, et al. 1991. J. Exp. Med. 173:1213. (IP)
- Adkins B, et al. 1988. Immunogenetics 27:180. (IHC)
- Surh CD, et al. 1992. J. Exp. Med. 176:611. (IHC)
- Goverman J, et al. 1997. Immunol. Today 18:204. (IHC)
- Dumont-Lagace M, 2014. J. Immunol. 192:2219. PubMed
- Kvell K, et al. 2010. PLoS One 5:e10701. (FC, IHC)
- Griewank K, et al. 2007. Immunity 27:751. (FC, IHC)
- Product Citations
-
- Padonou F, et al. 2022. EMBO Rep. 23:e53576. PubMed
- Fujimori S, et al. 2022. Elife. 11:. PubMed
- Li C, et al. 2018. Nat Commun. 1.073611111. PubMed
- Chen J, et al. 2021. Sci Adv. 7:. PubMed
- Giraud M, et al. 2014. Proc Natl Acad Sci U S A. 111:1491. PubMed
- Geng T, et al. 2022. Methods Mol Biol. 2585:71. PubMed
- Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
- Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
- Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
- Wells KL, et al. 2020. Elife. 9:. PubMed
- Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
- Guyon C, et al. 2020. eLife. 9:e52985.. PubMed
- Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
- Li J, et al. 2020. Development. . PubMed
- Yin S et al. 2019. Cancer cell. 35(2):283-296 . PubMed
- Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
- Yoshida H, et al. 2019. Cell. 176:897. PubMed
- Bansal K, et al. 2017. Nat Immunol. 18:263-273. PubMed
- Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
- Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
- RRID
- AB_313364 (BioLegend Cat. No. 108307) AB_313365 (BioLegend Cat. No. 108308)
- Structure
- Aminopeptidase A, 140 kD
- Distribution
-
B progenitors, bone marrow stromal lines, thymic dendritic cells, cortical epithelial cells
- Function
- Aminopeptidase, may function in IL-7-driven proliferation of B cell precursors
- Cell Type
- B cells, Dendritic cells, Epithelial cells
- Biology Area
- Immunology
- Antigen References
-
1. Goverman J, et al. 1997. Immunol. Today 18:204.
2. Morse HC, et al. 1987. J. Exp. Med. 165:920.
3. Sherwood P, et al. 1990. Int. Immunol. 2:399.
4. Lin Q, et al. 1998. J. Immunol. 160:4681.
- Gene ID
- 13809 View all products for this Gene ID
- UniProt
- View information about Ly-51 on UniProt.org
