Alexa Fluor® 647 anti-mouse Ly-51 Antibody
- 产品名称：
- Alexa Fluor® 647 anti-mouse Ly-51 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 108311
- 产品应用：
- 108311
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- C57BL/6 mouse Pre-B lymphoma cell line (L1-2) plus Abelson murine leukemia virus-specific cytotoxic T-cell clones
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2 and immunohistochemical staining3-5, 7-8 of acetone-fixed frozen sections.
- Application References
(PubMed link indicates BioLegend citation) -
- Pillemer E, et al. 1984. P. Natl. Acad. Sci. USA 81:4434. (IP)
- Hardy RR, et al. 1991. J. Exp. Med. 173:1213. (IP)
- Adkins B, et al. 1988. Immunogenetics 27:180. (IHC)
- Surh CD, et al. 1992. J. Exp. Med. 176:611. (IHC)
- Goverman J, et al. 1997. Immunol. Today 18:204. (IHC)
- Dumont-Lagace M, 2014. J. Immunol. 192:2219. PubMed
- Kvell K, et al. 2010. PLoS One 5:e10701. (FC, IHC)
- Griewank K, et al. 2007. Immunity 27:751. (FC, IHC)
- Product Citations
-
- Fujimori S, et al. 2022. Elife. 11:. PubMed
- Ohigashi I, et al. 2021. Cell Rep. 36:109657. PubMed
- Dumont-Lagacé M, et al. 2014. J Immunol. 192:2219. PubMed
- Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
- Liang Z, et al. 2021. J Cell Physiol. 236:4725. PubMed
- Ohigashi I, et al. 2020. Cell Reports. 29(9):2901-2916.e6.. PubMed
- Gruver A, et al. 2009. J Endocrinol. 203:75. PubMed
- Gulati GS, et al. 2018. Nat Protoc. 13:1294. PubMed
- Tsukasaki M, et al. 2020. Cell Reports. 32(10):108124. PubMed
- Chen P, et al. 2017. Sci Rep. . 10.1038/s41598-017-16162-2. PubMed
- Kang X, et al. 2022. J Immunol Res. 2022:8118577. PubMed
- Zhang Q, et al. 2021. Front Cell Dev Biol. 9:655552. PubMed
- Lebel M?, et al. 2020. Nat Commun. 3.051388889. PubMed
- Dong X, et al. 2022. Front Immunol. 13:896472. PubMed
- St-Pierre C, et al. 2015. J Immunol. 195: 498 - 506. PubMed
- Zhang Q, et al. 2022. Commun Biol. 5:544. PubMed
- Menon S, et al. 2021. Nat Commun. 12:4640. PubMed
- Liang Z, et al. 2017. Autophagy. 14:505. PubMed
- Dumont-Lagacé M, et al. 2015. Sci Rep. 5: 12895. PubMed
- Liang Z, et al. 2022. iScience. 25:105233. PubMed
- RRID
- AB_2099614 (BioLegend Cat. No. 108311) AB_2099613 (BioLegend Cat. No. 108312)
- Structure
- Aminopeptidase A, 140 kD
- Distribution
-
B progenitors, bone marrow stromal lines, thymic dendritic cells, cortical epithelial cells
- Function
- Aminopeptidase, may function in IL-7-driven proliferation of B cell precursors
- Cell Type
- B cells, Dendritic cells, Epithelial cells
- Biology Area
- Immunology
- Antigen References
-
1. Goverman J, et al. 1997. Immunol. Today 18:204.
2. Morse HC, et al. 1987. J. Exp. Med. 165:920.
3. Sherwood P, et al. 1990. Int. Immunol. 2:399.
4. Lin Q, et al. 1998. J. Immunol. 160:4681.
- Gene ID
- 13809 View all products for this Gene ID
- UniProt
- View information about Ly-51 on UniProt.org
