PE anti-mouse PIR-A/B Antibody
- 产品名称：
- PE anti-mouse PIR-A/B Antibody
- 产品类别：
- 抗体
- 产品编号：
- 144103
- 产品应用：
- 144103
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant extracellular domains 1 and 2 of PIR-A
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume.? It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications for the relevant formats include: Western Blotting1 and immunohistochemical staining of frozen tissue sections3.
- Application References
(PubMed link indicates BioLegend citation) -
- Kubagawa H, et al. 1999. J. Exp. Med. 189:309. (WB)
- Nakamura A, et al. 2004. Nat. Immunol. 5:623. (FC)
- Masuda K, et al. 2005. EMBO J. 24:4052. (IHC)
- Product Citations
-
- Ye Y, et al. 2017. Sci Immunol. 2:17. PubMed
- RRID
- AB_11203371 (BioLegend Cat. No. 144103) AB_11204242 (BioLegend Cat. No. 144104)
- Structure
- Transmembrane proteins, six Ig-like domains. PIR-A associates with FcRγc, doesn't have functional motifs on its cytoplasmic domain. PIR-B contains 3 ITIM motifs on its cytoplasmic domain.
- Distribution
-
B cells, macrophages, dendritic cells, mast cells, eosinophils.
- Interaction
- PIR-A: FcRγc
PIR-B: Lyn, PTPN6/SHP-1, PTPN11/SHP-2 - Ligand/Receptor
- MHC class I, Nogo, MAG, OMgp.
- Cell Type
- B cells, Dendritic cells, Eosinophils, Macrophages, Mast cells, Neutrophils
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- MHC Antigens, Protein Kinases/Phosphatase
- Antigen References
-
1. Matsushita H, et al. 2011. J. Biol. Chem. 286:25739.
2. Arita K, et al. 2011. J. Immunol. 186:7050.
3. Takai T, et al. 2011. J. Biomed. Biotechnol. 2011:275302.
4. Nakamura A, et al. 2004. Nat. Immunol. 5:623.
- Gene ID
- 18722 View all products for this Gene ID 18733 View all products for this Gene ID
- UniProt
- View information about PIR-A/B on UniProt.org
