Ultra-LEAF™ Purified anti-mouse TNF-α
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse TNF-α
- 产品类别：
- 抗体
- 产品编号：
- 510805
- 产品应用：
- 510805
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- recombinant mouse TNF-α
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
ELISA Capture - Quality tested
ELISPOT Capture - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by?ELISA assay. For ELISA capture applications, a concentration range of 2.0 - 6.0 ?g/mL?is recommended. To obtain a linear standard curve, serial dilutions of mouse TNF-α recombinant protein ranging from 500 to 4 pg/mL?are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA or ELISPOT Capture: The purified 6B8 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP6-XT22 antibody (Cat. No. 506312) as the detecting antibody. The Ultra-LEAF? purified antibody is suggested for ELISPOT capture.
Note: For testing mouse TNF-a in serum, plasma or supernatant, BioLegend's ELISA Max? Sets (Cat. No. 430901 to 430906) are specially developed and recommended.
- RRID
- AB_2876739 (BioLegend Cat. No. 510805) AB_2876739 (BioLegend Cat. No. 510806)
- Structure
- TNF superfamily; dimer/trimer; 18-150 kD (Mammalian)
- Bioactivity
- Paracrine/endocrine mediator of inflammatory and immune functions; selectively cytotoxic for transformed cells; endothelial cell alterations; chemoattractant
- Cell Sources
- Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
- Cell Targets
- Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia
- Receptors
- TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
- Cell Type
- Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Beutler B, et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler B, et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey K, et al. 1993. Crit. Care Med. 21:S415.
- Regulation
- Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists,
- Gene ID
- 21926 View all products for this Gene ID
- UniProt
- View information about TNF-alpha on UniProt.org
