| 规格 | 价格 | 库存 |
|---|---|---|
| 25µg | ¥ 1836 | 1 |
FC - Quality tested
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.5 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional reported applications (for relevant formats) include: immunoprecipitation1,2 and blocking3,5 of OT-I T cell responses in vitro and incidence of cerebral malaria in vivo caused by P. berghei ANKA (PbA) infection. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 139104). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 139117 - 139122) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin < 0.01 EU/?g).
Clone 6A6 antibody reacts only with the C57BL/6 allele of BTLA but not the BALB/c allele of BTLA.
BTLA is expressed on a wide number of lymphocytes in mice. It is most highly expressed on B cells, followed by CD4+ T cells, lower expression on CD8+ T cells, macrophages, dendritic cells, NKT cells, and NK cells.
1. Liu X, et al. 2009. J?Immunol. 182:4516.
2. Miller ML, et al. 2009. J?Immunol. 183:32.
3. Sun Y, et al. 2009. J?Immunol. 183:1946.
4. Vendel AC, et al. 2009. J?Immunol. 182:1509.
5. Watanabe N, et al. 2003. Nat?Immunol. 4:670.