Purified anti-human CD164 Antibody
- 产品名称：
- Purified anti-human CD164 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 324802
- 产品应用：
- 324802
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- T-47D breast carcinoma line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
ICC?- Verified
WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per 106 cells in 100 ?L?volume or 100 ?L?of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting2,3 under non-reducing conditions (detects an 80-100 kD protein as well as a high molecular weight aggregate of approximately 220 kD), and immunofluorescence3.
- Application References
(PubMed link indicates BioLegend citation) -
- Watt SM, et al. 1998. Blood 92:849.
- Watt SM, et al. 2000. Blood 95:3113.
- Doyonnas R, et al. 2000. J. Immunol. 165:840.
- Vogel W, et al. 2002. Haematologica 88:126.
- Product Citations
-
- Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed
- Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
- Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
- RRID
- AB_756020 (BioLegend Cat. No. 324802)
- Structure
- Single pass transmembrane protein with short cytoplasmic tail. Highly N- and O-glycosylated, contains sialic acid and a Ser-Gly motif that may serve as an attachment for a glycosaminoglycan side chain. Three splice variants have been reported with apparen
- Distribution
-
Expressed in bone marrow, bone marrow stromal cells, and CD34+ hematopoietic cells myeloid and erythroid progenitors; activated basophils. Expressed on a variety of carcinomas and leukemic cells. Also expressed in the small intestine, colon, l
- Function
- Plays a role in cell adhesion and proliferation, negative signaling molecule for hematopoietic progenitor cells. Has also been reported to be involved in myogenic differentiation and cancer metastasis
- Cell Type
- Basophils, Hematopoietic stem and progenitors, Leukemia
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Zannettino ACW, et al. 1998. Blood 92:2613.
2. Havens AM, et al. 2006. BMC Cancer 6:195.
3. Lee YN, et al. 2001. Mol. Cell. Biol. 21:7696.
- Gene ID
- 8763 View all products for this Gene ID
- UniProt
- View information about CD164 on UniProt.org
