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Biotin anti-6-His Epitope Tag Antibody (Previously
产品名称:
Biotin anti-6-His Epitope Tag Antibody (Previously
产品类别:
抗体
产品编号:
906103
产品应用:
906103
[价格]
规格 价格 库存
100µL ¥ 5028 1

产品详情

Product Details

Verified Reactivity
6xHis Tag
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB - Quality tested
ICC, IP - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 ?g per mL?(1:1000-1:2000 dilution).?For immunocytochemistry, a concentration range of 0.5 - 1.0 μg/mL?(1:1000-1:2000 dilution) is recommended.?For immunoprecipitation, the suggested use of this reagent is 1:150 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column.

Application References

(PubMed link indicates BioLegend citation)
  1. Deinert K, et al. 2001. J Biol Chem. 276:6000. (WB)
  2. Kojima H, et al. 2001. J Biol Chem. 276:19238. (IF) PubMed
  3. Lapinski PE, et al. 2000. J Biol Chem. 275:6831. (WB)
  4. Bartsch D, et al. 1998. Cell. 95:211. (IF)
Product Citations
  1. Kojima H, et al. 2001. J Biol Chem. 276:19238-19243. PubMed
RRID
AB_2565057 (BioLegend Cat. No. 906103)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about 6-His on UniProt.org

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