Biotin anti-6-His Epitope Tag Antibody (Previously
- 产品名称:
- Biotin anti-6-His Epitope Tag Antibody (Previously
- 产品类别:
- 抗体
- 产品编号:
- 906103
- 产品应用:
- 906103
- Verified Reactivity
- 6xHis Tag
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH.
- Formulation
- Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
WB - Quality tested
ICC, IP - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 ?g per mL?(1:1000-1:2000 dilution).?For immunocytochemistry, a concentration range of 0.5 - 1.0 μg/mL?(1:1000-1:2000 dilution) is recommended.?For immunoprecipitation, the suggested use of this reagent is 1:150 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column.
- Application References
(PubMed link indicates BioLegend citation) -
- Deinert K, et al. 2001. J Biol Chem. 276:6000. (WB)
- Kojima H, et al. 2001. J Biol Chem. 276:19238. (IF) PubMed
- Lapinski PE, et al. 2000. J Biol Chem. 275:6831. (WB)
- Bartsch D, et al. 1998. Cell. 95:211. (IF)
- Product Citations
-
- Kojima H, et al. 2001. J Biol Chem. 276:19238-19243. PubMed
- RRID
- AB_2565057 (BioLegend Cat. No. 906103)