Alexa Fluor® 488 anti-mouse CD49e Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2748	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

(C57BL/6 x A/J)F₁ mouse mast cell line

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining^6,7 of acetone-fixed frozen sections, blocking of cell-cell adhesion^1-5, inhibition of TNF-?₁ costimulated T cell proliferation³, and costimulation of T cell proliferation by cross-linked 5H10-27 antibody³. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 103817 and 103818).

Application References (PubMed link indicates BioLegend citation)

Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
Halvorson MJ, et al. 1995. J. Immunol. 155:4567. (Block)
Rich S, et al. 1996. J. Immunol. 157:2916. (Block Costim))
Uhlenkott CE, et al. 1996. Clin. Exp. Metastasis 14:125. (Block)
Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block)
Yang JT, et al. 1996. Mol. Biol. Cell. 7:1737. (IHC)
Cuklerman E, et al. 2001. Science 294:1708. (IHC)
Jia W, et al. 2005. Blood 106:3854. (FC)

Product Citations

Yan Q, et al. 2017. PLoS One. 12(6):e0179033. PubMed
Duru N, et al. 2016. Sci Rep. 6:39572. PubMed
Le AH, et al. 2021. Journal of Cell Biology. 220(9):. PubMed

RRID

AB_528839 (BioLegend Cat. No. 103810)

Antigen Details

Structure: Integrin family, associates with integrin β₁, 135 kD
Distribution: Thymocytes, activated T cells, mast cells
Function: Adhesion, costimulation
Ligand/Receptor: Fibronectin
Cell Type: Mast cells, Mesenchymal Stem Cells, T cells, Thymocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1994 Blood Cells 20:25.
3. Hemler ME. 1990. Annu. Rev. Immunol. 8:365.
Gene ID: 16402 View all products for this Gene ID
UniProt: View information about CD49e on UniProt.org

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