Purified anti-mouse CD8a Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 996	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Concanavalin A-stimulated BALB/c splenic T cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
ICC - Verified
IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: partial inhibition of T-cell responses to IL-2 and cytotoxic induction of splenic T cells¹, immunohistochemistry of acetone-fixed frozen sections², and immunofluorescence. The 5H10-1 antibody competes with 53-6.7 antibody (Cat. No. 100702) for binding to thymocytes¹.

Clone 5H10-1 is not recommended for immunohistochemistry of formalin-fixed paraffin sections.

Application References (PubMed link indicates BioLegend citation)

Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557.
Frei K, et al. 1997. J. Exp. Med. 185:2177.

Product Citations

Han C, et al. 2021. Cell Reports. 34(6):108706. PubMed
Hu J, et al. 2019. Mol Ther Nucleic Acids. 16:650. PubMed
Scutts SR, et al. 2018. Cell Rep. 25:1953. PubMed

RRID

AB_312762 (BioLegend Cat. No. 100801) AB_312763 (BioLegend Cat. No. 100802)

Antigen Details

Structure: Ig superfamily, CD8α chain, 34 kD
Distribution: Most thymocytes, T cell subset, some NK cells
Function: Co-receptor for TCR
Ligand/Receptor: MHC class I molecule
Cell Type: Dendritic cells, NK cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.
Gene ID: 12525 View all products for this Gene ID
UniProt: View information about CD8a on UniProt.org

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