FITC anti-mouse CD8a Antibody
- 产品名称：
- FITC anti-mouse CD8a Antibody
- 产品类别：
- 抗体
- 产品编号：
- 100803
- 产品应用：
- 100803
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Concanavalin A-stimulated BALB/c splenic T cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: partial inhibition of T-cell responses to IL-2 and cytotoxic induction of splenic T cells1, and immunohistochemistry2 of acetone-fixed frozen sections. The 5H10-1 antibody competes with 53-6.7 antibody (Cat. No. 100702) for binding to thymocytes1.
Clone 5H10-1 is not recommended for immunohistochemistry of formalin-fixed paraffin sections.
- Application References
(PubMed link indicates BioLegend citation) -
- Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557.
- Frei K, et al. 1997. J. Exp. Med. 185:2177.
- Korrer MJ, et al. 2014. PLoS One. 9:91370. PubMed
- Nakajima A, et al. 2014. PLoS One. 9:105904. PubMed
- Product Citations
-
- McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
- Bhatt K, et al. 2018. mSphere. 3:e00352. PubMed
- Shrivastava T, et al. 2021. Front Immunol. 12:641447. PubMed
- Shin JE, et al. 2021. Heliyon. 7:e08433. PubMed
- Nakajima A, et al. 2014. PLoS One. 9:105904. PubMed
- Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
- Wang F, et al. 2019. MAbs. 12:1685350. PubMed
- Moutuou MM, et al. 2020. Methods Mol Biol. 2111:267. PubMed
- Korrer M, Routes J 2014. PLoS One. 9:91370. PubMed
- Derada Troletti C, et al. 2021. Cell Reports. 35(9):109201. PubMed
- Wu J, et al. 2021. STAR Protoc. 2:101022. PubMed
- Cá B, et al. 2019. Front Microbiol. 1.876388889. PubMed
- Johannsen A, et al. 2010. J Immunol. 185:3445. PubMed
- Suhail A, et al. 2019. Cell Rep. 29:3522. PubMed
- RRID
- AB_312764 (BioLegend Cat. No. 100803) AB_312765 (BioLegend Cat. No. 100804)
- Structure
- Ig superfamily, CD8α chain, 34 kD
- Distribution
-
Most thymocytes, T cell subset, some NK cells
- Function
- Co-receptor for TCR
- Ligand/Receptor
- MHC class I molecule
- Cell Type
- Dendritic cells, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.
- Gene ID
- 12525 View all products for this Gene ID
- UniProt
- View information about CD8a on UniProt.org
