PerCP/Cyanine5.5 anti-human CD137L (4-1BB Ligand)
- 产品名称：
- PerCP/Cyanine5.5 anti-human CD137L (4-1BB Ligand)
- 产品类别：
- 抗体
- 产品编号：
- 311518
- 产品应用：
- 311518
[价格]
| 规格 |
价格 |
库存 |
| 100tests |
￥ 4584 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 311504) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Gullo C,?et al. 2010.?PLoS One. 5:e10845. (FC)?PubMed
- RRID
- AB_2749936 (BioLegend Cat. No. 311517) AB_2749937 (BioLegend Cat. No. 311518)
- Structure
- TNF superfamily, 97 kD
- Distribution
-
Antigen presenting cells, activated B cells and T cells
- Function
- T cell proliferation, cytokine production
- Ligand/Receptor
- 4-1BB (CDw137)
- Cell Type
- Antigen-presenting cells, B cells, T cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Akiba H,?et al.?2000.?J. Exp. Med.?191:375.?
2. Pollak KE,?et al.?1995.?Eur. J. Immunol.?25:488.?
3. DeBenedette MA,?et al.?1997.?J. Immunol.?158:551.?
4. Goodwin RG,?et al.?1993.?Eur. J. Immunol.?23:2631.
- Gene ID
- 8744 View all products for this Gene ID
- UniProt
- View information about CD137L on UniProt.org
