PE/Dazzle™ 594 anti-human CD137L (4-1BB Liga-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1908	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Dazzle? 594 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC -?Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* PE/Dazzle? 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 311504) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).

Application References (PubMed link indicates BioLegend citation)

Gullo C,?et al. 2010.?PLoS One. 5:e10845. (FC)?PubMed

RRID

AB_2734285 (BioLegend Cat. No. 311515) AB_2783180 (BioLegend Cat. No. 311516)

Antigen Details

Structure: TNF superfamily, 97 kD
Distribution: Antigen presenting cells, activated B cells and T cells
Function: T cell proliferation, cytokine production
Ligand/Receptor: 4-1BB (CDw137)
Cell Type: Antigen-presenting cells, B cells, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Akiba H,?et al.?2000.?J. Exp. Med.?191:375.?
2. Pollak KE,?et al.?1995.?Eur. J. Immunol.?25:488.?
3. DeBenedette MA,?et al.?1997.?J. Immunol.?158:551.?
4. Goodwin RG,?et al.?1993.?Eur. J. Immunol.?23:2631.
Gene ID: 8744 View all products for this Gene ID
UniProt: View information about CD137L on UniProt.org

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