Ultra-LEAF™ Purified anti-mouse GITR Ligand
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse GITR Ligand
- 产品类别：
- 抗体
- 产品编号：
- 147407
- 产品应用：
- 147407
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additonal reported applications (for relevant formats of thes clone) include: inhibition of GITR-GITRL mediated T cell proliferation and reversing Treg-mediated suppression1.
The following patents apply to clone 5F1: U.S. Patent Serial No. 7,618,632 and Japan Patent Serial No. 4638876.
- Application References
(PubMed link indicates BioLegend citation) -
- Stephens GL, et al. 2004. J. Immunol. 173:5008. (Block)
- RRID
- AB_2832526 (BioLegend Cat. No. 147407) AB_2832527 (BioLegend Cat. No. 147408)
- Structure
- TNF superfamily member
- Distribution
-
B cells, macrophages, dendritic cells, endothelial cells
- Function
- Binds GITR receptor to costimulate T cell activation, proliferation, and cytokine production; inhibits the suppressive activities of CD4+CD25+ Tregs
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Macrophages, Tregs
- Biology Area
- Immunology
- Antigen References
-
1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059.
2. Kim JD, et al. 2003. Genes Immun. 4:564.
3. Yu KY, et al. 2003. Biochem. Biophys. Res. Commun. 310:433.
4. Shin HH, et al. 2002. Cytokine 19:187.
- Gene ID
- 240873 View all products for this Gene ID
- UniProt
- View information about GITR Ligand on UniProt.org
