TotalSeq™-A0880 anti-mouse GITR Ligand Antib-上海雅吉生物科技有限公司

规格	价格	库存
10µg	￥ 4428	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA

Preparation

The antibody was purified by chromatography and conjugated with TotalSeq?-A oligomer under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

PG - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq?-A antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 ?g per million cells in 100 ?L volume. Refer to the corresponding TotalSeq? protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq? products. For example, for any technology platform Buyer uses with TotalSeq?, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq? with that platform.

Application Notes

Additonal reported applications (for relevant formats of thes clone) include: inhibition of GITR-GITRL mediated T cell proliferation and reversing Treg-mediated suppression¹.

The following patents apply to clone 5F1: U.S. Patent Serial No. 7,618,632 and Japan Patent Serial No. 4638876.

Additional Product Notes

TotalSeq? reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The barcode flanking sequences are CCTTGGCACCCGAGAATTCCA (PCR handle), and BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A (capture sequence). B represents either C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Scientific Poster Library.

Application References (PubMed link indicates BioLegend citation)

Stephens GL, et al. 2004. J. Immunol. 173:5008. (Block)

RRID

AB_2832525 (BioLegend Cat. No. 147405)

Antigen Details

Structure: TNF superfamily member
Distribution: B cells, macrophages, dendritic cells, endothelial cells
Function: Binds GITR receptor to costimulate T cell activation, proliferation, and cytokine production; inhibits the suppressive activities of CD4⁺CD25⁺ Tregs
Cell Type: B cells, Dendritic cells, Endothelial cells, Macrophages, Tregs
Biology Area: Immunology
Antigen References: 1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059.
2. Kim JD, et al. 2003. Genes Immun. 4:564.
3. Yu KY, et al. 2003. Biochem. Biophys. Res. Commun. 310:433.
4. Shin HH, et al. 2002. Cytokine 19:187.
Gene ID: 240873 View all products for this Gene ID
UniProt: View information about GITR Ligand on UniProt.org

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