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Purified anti-human CD193 (CCR3) Antibody
产品名称:
Purified anti-human CD193 (CCR3) Antibody
产品类别:
抗体
产品编号:
310702
产品应用:
310702
[价格]
规格 价格 库存
100µg ¥ 2196 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 ?g per million cells in 100 ?l volume.?For immunohistochemistry on paraffin-embedded tissue, a concentration range of 5.0 - 10.0 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: The 5E8 antibody is useful for immunofluorescent staining and flow cytometric analysis of CCR3 expression.

It has been observed that the 5E8 antibody clone can interact with PE/Cy7 antibody conjugates during multi-color staining, potentially leading to unwanted staining. This interaction can be resolved by sequentially staining with the 5E8 antibody first and then followed by the PE/Cy7 conjugate of interest.

Application References

(PubMed link indicates BioLegend citation)
  1. Beauvillian C, et al. 2011. Blood 117:1196. PubMed
Product Citations
  1. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  2. Olin A, et al. 2018. Cell. 174:1277. PubMed
  3. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  4. Lakshmikanth T, et al. 2020. Cell Reports. 32(3):107923. PubMed
  5. Beauvillain C, et al. 2011. Blood. 117:1196. PubMed
  6. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
RRID
AB_345394 (BioLegend Cat. No. 310702)

Antigen Details

Structure
G-protein coupled seven transmembrane domain receptor, 356 amino acids
Distribution

Eosinophils, basophils, mast, mononuclear phagocytes, platelets, CD34+ hematopoietic progenitor, Th2, keratinocytes

Function
Co-receptor for HIV-1 and HIV-2, allergy
Receptors
Eotaxin, eotaxin-2, eotaxin-3
Cell Type
Eosinophils, Erythrocytes, Hematopoietic stem and progenitors, Macrophages, Mast cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Gerard W, et al. 1996. J. Exp. Med. 183:2437.
2. Uguccioni C, et al. 1997. J. Clin. Invest. 100:1137.
3. Sallusto F, et al. 1997. Science. 277:2005.
4. Loetscher P, et al. 2001. J. Biol. Chem. 276:2986.

Regulation
Upregulated by high affinity Fc IgE receptor ligation (mast cells), RANTES (keratinocytes), IFNg (monocytes, neutrophils), HIV tat protein (basophils), IL-3, IL-5 and GM-CSF (CD34+ progenitor cells), IL-2 and IL-4(T cells). Downregulated by IL-
Gene ID
1232 View all products for this Gene ID
UniProt
View information about CD193 on UniProt.org

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