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Alexa Fluor® 647 anti-mouse Blimp-1 Antibody
产品名称:
Alexa Fluor® 647 anti-mouse Blimp-1 Antibody
产品类别:
抗体
产品编号:
150003
产品应用:
150003
[价格]
规格 价格 库存
25µg ¥ 1980 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Amino acids 255-395 from mouse Blimp-1 fused with GST.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed
  2. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  3. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  4. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  5. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  6. Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
  7. Roy K, et al. 2019. Immunity. 50:616. PubMed
  8. Zhu H, et al. 2019. Nat Commun. 10:1084. PubMed
  9. Cho S, et al. 2018. Nat Commun. 9:2757. PubMed
  10. Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed
  11. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  12. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
RRID
AB_2565617 (BioLegend Cat. No. 150003) AB_2565618 (BioLegend Cat. No. 150004)

Antigen Details

Structure
Five Kruppel-type zinc finger domains, 98 kD.
Distribution
Plasma cells, CD4+ and CD8+ effector/memory T cells, and natural killer cells.
Function
Terminal differentiation of B cells, effector/memory T cells, role in natural killer cells (NK) and T cell homeostasis, and repressor of BCL6 and c-Myc.
Cell Type
Plasma cells, T cells, NK cells, B cells
Biology Area
Cell Biology, Immunology, Transcription Factors
Antigen References

1. Nakaki F, et al. 2013. Nature 501:222.
2. Crotty S, et al. 2010. Nat. Immunol. 11:114.
3. Zhao WL, et al. 2008. Blood 111:3867.
4. Climmino L, et al. 2008. J. Immunol. 181:2338.
5. Martins, G and Clarne, K. 2008. Annu. Rev. Immunol. 26:133.

Gene ID
12142 View all products for this Gene ID
UniProt
View information about Blimp-1 on UniProt.org

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