APC anti-mouse/human SEMA4A Antibody
- 产品名称：
- APC anti-mouse/human SEMA4A Antibody
- 产品类别：
- 抗体
- 产品编号：
- 148405
- 产品应用：
- 148405
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Mouse SEMA4A-Fc proteins
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for relevant formats) include: ELISA1.
- Application References
(PubMed link indicates BioLegend citation) -
- Kumanogoh A. et al. 2005. Immunity. 22:305. (ELISA)
- Product Citations
-
- Ruffin AT, et al. 2021. Nat Commun. 12:3349. PubMed
- RRID
- AB_2728180 (BioLegend Cat. No. 148405) AB_2728181 (BioLegend Cat. No. 148406)
- Structure
- Single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a SEMA domain.
- Distribution
-
Dendritic cells, T cells, B cells, brain, lung, kindney, spleen and testis
- Function
- An activator in T-cell-mediated immune response. Suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. It is also involved in guidance of axonal migration during neuronal development.
- Cell Type
- Dendritic cells, T cells, B cells, Tregs
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules
- Antigen References
-
1. Kumanogoh A, et al. 2002. Nature. 419:629.?
2. Kumanogoh A, et al. 2005. Immunity. 22:305.?
3. Toyofuku T, et al. 2007. EMBO J. 26:1373.?
4. Delgoffe GM, et al. 2013. Nature. 501:252.
- Gene ID
- 20351 View all products for this Gene ID 64218 View all products for this Gene ID
- UniProt
- View information about SEMA4A on UniProt.org
