Go-ChIP-Grade™ Purified anti-Histone H4 Mono
- 产品名称：
- Go-ChIP-Grade™ Purified anti-Histone H4 Mono
- 产品类别：
- 抗体
- 产品编号：
- 828003
- 产品应用：
- 828003
- Verified Reactivity
- Human, Rat, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a synthetic peptide conjugated to KLH containing methylated lysine 20 of human Histone H4.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ChIP - Quality tested
IHC-P, WB - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:100 by volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a dilution range of 1:100 - 1:200?is suggested. For Western blotting, the suggested use of this reagent is 0.1 ?g/mL (1:5000).? It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
25 ?g and?100 ?g of Go-ChIP-Grade? Purified Antibody can be used for 2-5 or 10-20 immunoprecipitations, respectively, at the recommended dilutions.
- Application References
(PubMed link indicates BioLegend citation) -
- Shirakata Y, et al. 2014. J. Reprod. Dev. 60:383. (IHC-P)
- RRID
- AB_2632897 (BioLegend Cat. No. 828003) AB_2632843 (BioLegend Cat. No. 828004)
- Structure
- Histone H4 is a 103 amino acid protein with an apparent molecular mass of 12 kD
- Distribution
-
Nucleus.
- Function
- Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Histone proteins H3 and H4 bind to form a H3-H4 dimer, two of these H3-H4 dimers combine to form a tetramer. This tetramer further combines with two H2a-H2b dimers to form the compact Histone octamer core.
- Interaction
- Various transcription factors and nuclear protein.
- Cell Type
- Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Stem Cells, Transcription Factors
- Antigen References
-
1. Chen HM, et al. 2015. Epigenetics and Dermatology. 409:441.
2. Wang Z, et al. 2008. Nat. Genet. 40:897.
3. Beck DB, et al. 2012. Genes Dev. 26:325.
- Gene ID
- 8359 View all products for this Gene ID
- UniProt
- View information about Histone H4 Monomethyl Lys20 on UniProt.org
