Purified anti-human CD90 (Thy1) Antibody
- 产品名称：
- Purified anti-human CD90 (Thy1) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 328101
- 产品应用：
- 328101
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Pigtailed Macaque, Rhesus, Pig
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- HEL cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF? - Verified
IHC-F, ICC, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone 5E10 recognizes an epitope on Thy-1 independent of its glycosylation, but is abolished under reducing conditions.4?Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation1, and immunofluorescence3.
- Application References
(PubMed link indicates BioLegend citation) -
- Craig W, et al. 1993. J. Exp. Med. 177:1331. (IP)
- Gundlach CW 4th, et al. 2011. Bioconjug. Chem. 22:1706. (Pig Reactivity)
- Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF)
- Bradley JE, et al. 2013. Lab Invest. 93:365. (Epitope)
- Donnenberg VS, et al. 2010. Cytometry B. Clin. Cytom. 5:287. (IHC)
- Product Citations
-
- Mendt M, et al. 2018. JCI Insight. 9:1523. PubMed
- Tornack J, et al. 2017. PLoS One. 12:e0169119. PubMed
- Slamecka J, et al. 2017. Cell Cycle. 17:330. PubMed
- Floy ME, et al. 2022. STAR Protoc. 3:101275. PubMed
- Fitzgerald W, et al. 2018. Am J Reprod Immunol. 80:e12860. PubMed
- Azari S, et al. 2021. MBio. 12:e0184921. PubMed
- Ga?án-Gómez I, et al. 2022. Nat Med. . PubMed
- Lin J, et al. 2019. J Immunol Res. 2019:6929286. PubMed
- Han L, et al. 2019. Haematologica. 10.3324/haematol.2018.205534. PubMed
- Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
- Ran LJ, et al. 2018. Mol Med Rep. 18:723. PubMed
- Crawford LB, et al. 2021. J Virol. 95:. PubMed
- Li Z, et al. 2020. J Clin Lab Anal. 34:e23155. PubMed
- RRID
- AB_940390 (BioLegend Cat. No. 328101) AB_940393 (BioLegend Cat. No. 328102)
- Structure
- 25-35 kD glycoprotein, Ig superfamily
- Distribution
-
Subset of CD34+ hematopoietic stem cells, subset of fetal thymocytes, subset of fetal liver cells, fibroblast, activated endothelial cells, neurons and some leukemia cell lines
- Function
- Regulate hematopoiesis and neural cell growth, cell adhesion
- Ligand/Receptor
- β3 integrin, β2 integrin
- Cell Type
- Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Leukemia, Mesenchymal Stem Cells, Neurons, Thymocytes
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. McKenzie JL, et al. 1981. J. Immunol. 126:843.
2. Avalos AM, et al. 2002. Biol. Res. 35:231.
3. Wetzel A, et al. 2004. J. Immunol. 172:3850.
- Gene ID
- 7070 View all products for this Gene ID
- UniProt
- View information about CD90 on UniProt.org
