Brilliant Violet 421™ anti-human CD90 (Thy1)
- 产品名称：
- Brilliant Violet 421™ anti-human CD90 (Thy1)
- 产品类别：
- 抗体
- 产品编号：
- 328121
- 产品应用：
- 328121
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2184 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Pigtailed Macaque, Rhesus, Pig
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- HEL cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Clone 5E10 recognizes an epitope on Thy-1 independent of its glycosylation, but is abolished under reducing conditions.4?Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation1, and immunofluorescence3.
- Application References
(PubMed link indicates BioLegend citation) -
- Craig W, et al. 1993. J. Exp. Med. 177:1331. (IP)
- Gundlach CW 4th, et al. 2011. Bioconjug. Chem. 22:1706. (Pig Reactivity)
- Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF)
- Bradley JE, et al. 2013. Lab Invest. 93:365. (Epitope)
- Donnenberg VS, et al. 2010. Cytometry B. Clin. Cytom. 5:287. (IHC)
- Product Citations
-
- Gomzikova MO, et al. 2019. Cells. 0.440972222. PubMed
- Lal A, et al. 2021. Nat Commun. 1507:12. PubMed
- Nafria M, et al. 2020. Cell Rep. 31:107691. PubMed
- Psaila B, et al. 2020. Mol Cell. 78:477. PubMed
- Roy A, et al. 2021. Cell Rep. 36:109698. PubMed
- Louka E, et al. 2021. Journal of Experimental Medicine. 218(2):. PubMed
- Audigé A, et al. 2017. BMC Immunology. 10.1186/s12865-017-0209-9. PubMed
- Rodriguez-Meira A, et al. 2020. STAR Protoc. 1:100125. PubMed
- Tamai K, et al. 2022. Cell Rep Methods. 2:100314. PubMed
- Busch A, et al. 2020. Int J Mol Sci. 21:. PubMed
- Nafria M, et al. 2020. STAR Protoc. 1:100130. PubMed
- Crawford LB, et al. 2021. J Virol. 95:. PubMed
- Brandau S, et al. 2014. PLoS One. 9:106903. PubMed
- Rodriguez‐Meira A et al. 2019. Molecular cell. 73(6):1292-1305 . PubMed
- RRID
- AB_10933261 (BioLegend Cat. No. 328121) AB_2561420 (BioLegend Cat. No. 328122)
- Structure
- 25-35 kD glycoprotein, Ig superfamily
- Distribution
-
Subset of CD34+ hematopoietic stem cells, subset of fetal thymocytes, subset of fetal liver cells, fibroblast, activated endothelial cells, neurons and some leukemia cell lines
- Function
- Regulate hematopoiesis and neural cell growth, cell adhesion
- Ligand/Receptor
- β3 integrin, β2 integrin
- Cell Type
- Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Leukemia, Mesenchymal Stem Cells, Neurons, Thymocytes
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. McKenzie JL, et al. 1981. J. Immunol. 126:843.
2. Avalos AM, et al. 2002. Biol. Res. 35:231.
3. Wetzel A, et al. 2004. J. Immunol. 172:3850.
- Gene ID
- 7070 View all products for this Gene ID
- UniProt
- View information about CD90 on UniProt.org
