APC anti-human MCP-1 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1644	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant human MCP-1

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Red Laser (633 nm)

Application Notes

ELISA or ELISPOT Detection¹: The biotinylated 5D3-F7 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified 2H5 antibody (Cat. No. 505902/505906) as the capture antibody.
ELISA or ELISPOT Capture: The purified 5D3-F7 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 2H5 antibody (Cat. No. 505908) as the detection antibody. The LEAF? purified antibody (Cat. No. 502607) is suggested for ELISPOT capture.
Additional reported applications (for the relevant formats) include: intracellular flow cytometry², immunoprecipitation^1,3, Western blotting¹, and immunohistochemical staining¹.

Application References (PubMed link indicates BioLegend citation)

Peri, G., et al. 1994. J. Immunol. Meth. 174:249. (IP, IHC, WB)
Rezaie-Majd, A., et al. 2002. Arterioscler Thromb Vasc Biol. 22:1194. (ICFC)
Hirsch, A., et al. 1999. J. Virol. 73:404. (IP)

RRID

AB_2734488 (BioLegend Cat. No. 502611) AB_2734489 (BioLegend Cat. No. 502612)

Antigen Details

Structure: CC chemokine; dimer; 13.8 kD (Mammalian)
Bioactivity: Chemoattractant; regulate adhesion molecule expression, cytokine production in monocytes; proliferation/activation of CC-chemokine activated killer cells
Cell Sources: Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells
Cell Targets: Monocytes, basophils, T cells, NK cells, immature dendritic cells
Receptors: CCR2; CCR5; CCR10; D6; possibly L-CCR
Cell Type: Embryonic Stem Cells
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Bischoff S, et al. 1992. J. Exp. Med. 175:1271.
3. Charo I, et al. 1994. P. Natl. Acad. Sci. USA 91:2752.
4. Jiang Y, et al. 1992. J. Immunol. 148:2423.
Regulation: Upregulated by IL-1, TNF-α, PDGF, TGF-β
Gene ID: 6347 View all products for this Gene ID
UniProt: View information about CCL2 on UniProt.org

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