TotalSeq™-D0031 anti-human CD40 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
10µg	￥ 4296	1

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

Baboon, Chimpanzee, Squirrel Monkey

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA

Preparation

The antibody was purified by chromatography and conjugated with TotalSeq?-D oligomer under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

PG - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq?-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension.?For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 ?g per million cells in 100 ?L volume.?Refer to the corresponding TotalSeq? protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq? products. For example, for any technology platform Buyer uses with TotalSeq?, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq? with that platform.

Application Notes

Additional reported applications (for the relevant formats) include: costimulation of B cell proliferation¹, partial inhibition of CD40 binding to CD40L³, and B cell rescue from apoptosis¹. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays.

Additional Product Notes

TotalSeq?-D reagents are designed to profile protein expression at single cell level. The?Mission Bio Tapestri platform?and sequencer (e.g. Illumina analyzers) are required. Please contact?technical support?for more information, or visit?biolegend.com/totalseq/single-cell-dna

The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our?Application Technical Notes.

Application References (PubMed link indicates BioLegend citation)

Schlossman SF, et al. 1995. ed. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press.
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Pound JD, et al. 1999. Int. Immunol. 11:11.
Shey MS, et al. 2014. J Immunol. 192:4833. PubMed
Sondergaard JN, et al. 2014. Mol Immunol. 59:180. PubMed
Saiki O, et al. 1995. J Clin. Invest. 2: 510-4 (Costim)

RRID

AB_2924539 (BioLegend Cat. No. 334354)

Antigen Details

Structure: TNFR superfamily, type I glycoprotein, 48 kD
Distribution: B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts
Function: B cell differentiation, costimulation, isotype class-switching, rescues B cells from apoptosis
Ligand/Receptor: CD154 (CD40 ligand)
Cell Type: B cells, Dendritic cells, Endothelial cells, Fibroblasts, Macrophages
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881.
2. Foy T, et al. 1996. Annu. Rev. Immunol. 14:591.
Gene ID: 958 View all products for this Gene ID
UniProt: View information about CD40 on UniProt.org

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