Purified anti-human CD40 Antibody
- 产品名称：
- Purified anti-human CD40 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 334302
- 产品应用：
- 334302
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Baboon, Chimpanzee, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
Costim - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: costimulation of B cell proliferation1, partial inhibition of CD40 binding to CD40L3, and B cell rescue from apoptosis1. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman SF, et al. 1995. ed. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press.
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Pound JD, et al. 1999. Int. Immunol. 11:11.
- Shey MS, et al. 2014. J Immunol. 192:4833. PubMed
- Sondergaard JN, et al. 2014. Mol Immunol. 59:180. PubMed
- Saiki O, et al. 1995. J Clin. Invest. 2: 510-4 (Costim)
- Product Citations
-
- Silvin A, et al. 2020. Cell. 182:1401. PubMed
- Tanner MR, et al. 2019. Arthritis Res Ther. 21:6. PubMed
- Trotta A, et al. 2018. Front Immunol. 1.069444444. PubMed
- Mask E, et al. 2022. Front Cell Infect Microbiol. 12:880860. PubMed
- Heesters BA, et al. 2021. J Exp Med. 218:. PubMed
- Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed
- Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
- Warncke M, et al. 2012. J Immunol. 188:4405. PubMed
- Lee-Chang C, et al. 2021. J Exp Med. 218:. PubMed
- Zimmerman MG, et al. 2018. Cell Host Microbe. 24:731. PubMed
- RRID
- AB_1236384 (BioLegend Cat. No. 334302)
- Structure
- TNFR superfamily, type I glycoprotein, 48 kD
- Distribution
-
B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts
- Function
- B cell differentiation, costimulation, isotype class-switching, rescues B cells from apoptosis
- Ligand/Receptor
- CD154 (CD40 ligand)
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Fibroblasts, Macrophages
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881.
2. Foy T, et al. 1996. Annu. Rev. Immunol. 14:591.
- Gene ID
- 958 View all products for this Gene ID
- UniProt
- View information about CD40 on UniProt.org
