PE/Fire™ 810 anti-human CD40 Antibody
- 产品名称：
- PE/Fire™ 810 anti-human CD40 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 334355
- 产品应用：
- 334355
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2748 |
1 |
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Baboon, Chimpanzee, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Fire? 810 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Fire? 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm.
Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire? 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page. - Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: costimulation of B cell proliferation1, partial inhibition of CD40 binding to CD40L3, and B cell rescue from apoptosis1. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman SF, et al. 1995. ed. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press.
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Pound JD, et al. 1999. Int. Immunol. 11:11.
- Shey MS, et al. 2014. J Immunol. 192:4833. PubMed
- Sondergaard JN, et al. 2014. Mol Immunol. 59:180. PubMed
- Saiki O, et al. 1995. J Clin. Invest. 2: 510-4 (Costim)
- Structure
- TNFR superfamily, type I glycoprotein, 48 kD
- Distribution
-
B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts
- Function
- B cell differentiation, costimulation, isotype class-switching, rescues B cells from apoptosis
- Ligand/Receptor
- CD154 (CD40 ligand)
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Fibroblasts, Macrophages
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881.
2. Foy T, et al. 1996. Annu. Rev. Immunol. 14:591.
- Gene ID
- 958 View all products for this Gene ID
- UniProt
- View information about CD40 on UniProt.org
