PE/Cyanine7 anti-human CD40 Antibody
- 产品名称：
- PE/Cyanine7 anti-human CD40 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 334321
- 产品应用：
- 334321
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1692 |
1 |
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Baboon, Chimpanzee, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: costimulation of B cell proliferation1, partial inhibition of CD40 binding to CD40L3, and B cell rescue from apoptosis1. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays.
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman SF, et al. 1995. ed. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press.
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Pound JD, et al. 1999. Int. Immunol. 11:11.
- Shey MS, et al. 2014. J Immunol. 192:4833. PubMed
- Sondergaard JN, et al. 2014. Mol Immunol. 59:180. PubMed
- Saiki O, et al. 1995. J Clin. Invest. 2: 510-4 (Costim)
- Product Citations
-
- Carre C, et al. 2021. iScience. 24:102970. PubMed
- Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
- Ziebart A, et al. 2018. Oncotarget. 9:5834. PubMed
- Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
- Grandclaudon M, et al. 2019. Cell. 179:432. PubMed
- Nowak W, et al. 2020. EBioMedicine. 50:290-305.. PubMed
- van Doorn CLR, et al. 2021. Front Immunol. 12:712021. PubMed
- Rusconi M, et al. 2017. Sci Rep. 5.745138889. PubMed
- Mysore V, et al. 2021. Med (N Y). 2:1050. PubMed
- RRID
- AB_10643414 (BioLegend Cat. No. 334321) AB_10645472 (BioLegend Cat. No. 334322)
- Structure
- TNFR superfamily, type I glycoprotein, 48 kD
- Distribution
-
B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts
- Function
- B cell differentiation, costimulation, isotype class-switching, rescues B cells from apoptosis
- Ligand/Receptor
- CD154 (CD40 ligand)
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Fibroblasts, Macrophages
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881.
2. Foy T, et al. 1996. Annu. Rev. Immunol. 14:591.
- Gene ID
- 958 View all products for this Gene ID
- UniProt
- View information about CD40 on UniProt.org
