Purified anti-Notch1β Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3180	1

Product Details

Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH conjugated peptide corresponding to aa 1721-1745 of human Notch1 (VQSETVEPPPPAQLHFMYVAAAAFV)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested
Direct ELISA - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2 - 5 μg/ml is suggested. For Direct ELISA, the suggested use of this reagent is 0.05 - 0.1 ?g/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 5C12 has been tested by direct ELISA on Notch ? (N?) peptide (1-17aa) coated plates. It has not been evaluated in a sandwich ELISA format.
RRID: AB_2616643 (BioLegend Cat. No. 845402)

Structure: 2555 amino acids with an approximate molecular mass of 272 kD.
Distribution: Highly expressed in the brain, lung, and thymus (CD4^-CD8^- cells and CD4^-CD8⁺ cells). Lower levels of expression in spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney and heart is observed.
Function: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Affects the implementation of differentiation, proliferation and apoptotic programs.
Interaction: Notch1 is processed in a manner similar to APP, generating N? peptides (S4 cleavage) and the Notch intracellular domain (NICD, S3 cleavage) which translocates to the nucleus and acts as an essential regulator of transcription factors.
Ligand/Receptor: Receptor for the membrane ligands Jagged1, Jagged2, and Delta1 to regulate cell fate decisions.
Cell Type: Thymocytes
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Synaptic Biology
Molecular Family: Postsynaptic proteins
Antigen References: 1. Okochi M, et al. 2006. J. Biol. Chem. 281(12):7890.
2. Wanngren J, et al. 2012. J. Biol. Chem. 287(39):32640.
3. Tanveer R, et al. 2012. J. Biol. Chem. 287(41):34709.
Gene ID: 4851 View all products for this Gene ID
UniProt: View information about Notch1beta on UniProt.org