Purified anti-Caspase-1 Antibody
- 产品名称：
- Purified anti-Caspase-1 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 645101
- 产品应用：
- 645101
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Bacterial fusion protein (p20)
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1-2?g antibody per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
(PubMed link indicates BioLegend citation) -
- Kang SJ, et al. 2000. J Cell Biol 149:613
- Kang SJ, et al. 2002. Cell Death and Differentiation. 9:1115
- Product Citations
-
- Liu L, et al. 2017. J Crohns Colitis. 10.1093/ecco-jcc/jjw219. PubMed
- Zhu Q, et al. 2016. Am J Physiol Renal Physiol. 310: F621-F627. PubMed
- Reis AS, et al. 2020. Sci Adv. 6:eaax6346. PubMed
- Nastase MV, et al. 2016. The American Journal of Pathology. 186(5):1206-20. PubMed
- RRID
- AB_2068901 (BioLegend Cat. No. 645101) AB_2068900 (BioLegend Cat. No. 645102)
- Structure
- The whole length of Caspase-1 is a 404 amino acid polypeptide chain with MW of 45kd. The active Caspase -1 is a tetramer compariseing two subunits of 20Kd and 10Kd.
- Distribution
-
Predominantly in the cytoplasm, some localized to the external cell membrane.
- Function
- Caspase-1 is a mediator of the inflammatory response by cleaving the inactive pro-IL1 beta at Asp116/Ala 117 to generate the active 17k mature form of IL-1 beta.
- Ligand/Receptor
- Pro-IL1 beta
- Bioactivity
- IL-1beta converting enzyme (ICE)
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
- Antigen References
-
1. Wilson KP et al. 1994. Nature 370:251
2. Cohen GM:1997 Biochem J 326:1
- Gene ID
- 834 View all products for this Gene ID
- UniProt
- View information about Caspase-1 on UniProt.org
