Purified anti-PARP Antibody
- 产品名称：
- Purified anti-PARP Antibody
- 产品类别：
- 抗体
- 产品编号：
- 614301
- 产品应用：
- 614301
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant (partial), N-terminal 2/3 sequence of PARP
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
KO/KD-WB, ICC, IHC-P?- Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 ?g/mL (1:500-1:1000 dilution). For immunocytochemistry, a concentration range of 1?- 5?μg/mL is recommended. For immunohistochemistry, a concentration range of 5?- 10??g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody recognizes N-terminal short cleaved PARP after Staurosporine treatment at around 24kDa.
- Application References
(PubMed link indicates BioLegend citation) -
- Bogiatzi SI, et al. 2012. J Allergy Clin Immunol. 130:233. PubMed.
- Gu Y, et al. 2013. Acta Biochim Biophys Sin. PubMed
- Product Citations
-
- Nakaya M, et al. 2017. J Clin Invest. 127:383. PubMed
- Wang F, et al. 2020. Int J Oncol. 57:707. PubMed
- Bogiatzi S, et al. 2012. J Allergy Clin Immunol. 130:233. PubMed
- Gu Y, et al. 2013. Acta Biochim Biophys Sin. 45:904. PubMed
- RRID
- AB_2160595 (BioLegend Cat. No. 614301) AB_2299318 (BioLegend Cat. No. 614302)
- Structure
- PARP family, BRCT domain, homo- or hetero-dimer; 113 kD
- Distribution
-
Nuclear
- Function
- Molecular "nick sensor"; base excision repair, catalyzes poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture, DNA metabolism; protein modification may enhance or repress transcription
- Interaction
- Component of a base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. Heterodimerizes with PARP2, interacts with PARP3, modifies TATA-BP, YY1, Sp1, NF-B, p53 and others
- Modification
- Ribosylation by PARP2
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, DNA Repair/Replication, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Cherney B, et al. 1987. P. Natl. Acad. Sci. USA 84:8370.
2. Ikejima M, et al. 1990. J. Biol. Chem. 265:21907.
3. Ying W, et al. 2001. P. Natl. Acad. Sci. USA 98:12227.
4. Noel G, et al. 2003. BMC Cell Biol. 4:7.
- Regulation
- Poly(ADP-ribose) glycohydrolase removes ribose chains, allows quick, transient ribosylation of proteins
- Gene ID
- 142 View all products for this Gene ID
- UniProt
- View information about PARP on UniProt.org
