Purified anti-MAPK14 Antibody
- 产品名称:
- Purified anti-MAPK14 Antibody
- 产品类别:
- 抗体
- 产品编号:
- 602651
- 产品应用:
- 602651
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human MAPK14 protein fragments
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P, ICC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 ?g/mL.?For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0??g/mL is suggested.?For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
For ICC, we recommend 4% PFA fixation followed by permeabilization with ice cold methanol. We do not recommend 4% PFA fixation followed by permeabilization with 0.5% Triton-X, or fixation/permeabilization with ice cold methanol alone.??
- RRID
- AB_2910477 (BioLegend Cat. No. 602651) AB_2910477 (BioLegend Cat. No. 602652)
- Structure
- MAPK14 is a 360 amino-acid protein with a predicted molecular weight of 41 kD.
- Distribution
-
MAPK14 is widely expressed in the cytoplasm and nucleus of most tissues.
- Function
- MAPK14 is a serine/threonine kinase.
- Interaction
- MAPK14 phosphorylates myriad targets including transcription factors, other kinases, enzymes, and histone proteins.
- Biology Area
- Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
- Canovas B, Nebreda A. 2021. Nat?Rev?Mol?Cell Biol. 22:346-366.
- Gene ID
- 1432 View all products for this Gene ID
- UniProt
- View information about MAPK14 on UniProt.org