Pacific Blue™ anti-human CD34 Antibody
- 产品名称：
- Pacific Blue™ anti-human CD34 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 343511
- 产品应用：
- 343511
- Verified Reactivity
- Human
- Reported Reactivity
- Cynomolgus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
The 581 antibody recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press.
- Felschow DM, et al. 2001. Blood 97:3768.
- Rudin CE, et al. 1997. Br. J. Haematol. 97:488.
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC)
- Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF)
- Product Citations
-
- Xu H, et al. 2019. Cell Stem Cell. 24:566. PubMed
- Qiu J, et al. 2020. Cell Rep. 33:108465. PubMed
- Mitchell K, et al. 2018. J Exp Med. 215:1709. PubMed
- Queckborner S, et al. 2020. Stem Cell Res Ther. 11:15. PubMed
- Wu Y, et al. 2019. Nat Med. 25:776. PubMed
- Bhatwadekar A, et al. 2015. Diabetes. 64: 4226 - 4237. PubMed
- Van Egeren D, et al. 2021. Cell Stem Cell. 28(3):514-523.e9. PubMed
- Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed
- Velázquez–Avila M, et al. 2019. Leukemia. 33:1337. PubMed
- Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
- Zeng J, et al. 2020. Nat Med. 26:535. PubMed
- Pellin D, et al. 2019. Nat Commun. 10:2395. PubMed
- Bhatwadekar A, et al. 2010. Diabetes. 59:2010. PubMed
- Roerden M, et al. 2021. Cancers. 13(11):. PubMed
- Pribyl M, et al. 2020. Mol Oncol. 2.252083333. PubMed
- Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
- Ueda K, et al. 2021. Cancer Cell. 39(4):529-547.e7. PubMed
- Jarajapu Y, et al. 2013. Diabetes. 62:1258. PubMed
- Yang C, et al. 2019. Nat Commun. 10:3931. PubMed
- RRID
- AB_1877198 (BioLegend Cat. No. 343511) AB_1877197 (BioLegend Cat. No. 343512)
- Structure
- 105-120 kD single chain mucin-like glycoprotein
- Distribution
-
Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts
- Function
- Cell adhesion
- Ligand/Receptor
- L-selectin, E-selectin
- Cell Type
- Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Krause DS, et al. 1996. Blood 87:1.
2. Puri KD, et al. 1995. J. Cell Biol. 131:261.
3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey.
- Gene ID
- 947 View all products for this Gene ID
- UniProt
- View information about CD34 on UniProt.org
