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APC/Cyanine7 anti-human CD34 Antibody
产品名称:
APC/Cyanine7 anti-human CD34 Antibody
产品类别:
抗体
产品编号:
343513
产品应用:
343513
[价格]
规格 价格 库存
25tests ¥ 1836 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 581 antibody recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press.
  2. Felschow DM, et al. 2001. Blood 97:3768.
  3. Rudin CE, et al. 1997. Br. J. Haematol. 97:488.
  4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  5. Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC)
  6. Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF)
Product Citations
  1. Takayama N, et al. 2020. Cell Stem Cell. 28(3):488-501.e10. PubMed
  2. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  3. Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed
  4. Shukla S, et al. 2021. Nat Chem Biol. 17:784. PubMed
  5. Montefiori LE, et al. 2021. Cancer Discov. 11:2846. PubMed
  6. Mori Y, et al. 2015. Proc Natl Acad Sci U S A. 112: 9638-9643. PubMed
  7. Nguyen MAT, et al. 2020. Immunohorizons. 0.465277778. PubMed
  8. Lal A, et al. 2021. Nat Commun. 1507:12. PubMed
  9. Bigley V, et al. 2011. J Exp Med. 208:227. PubMed
  10. Zhou X, et al. 2017. J Vasc Surg. 65:1161. PubMed
  11. Olesinski EA, et al. 2021. STAR Protocols. 2(2):100461. PubMed
  12. Dorrell C, et al. 2016. Nat Commun. 7:11756. PubMed
  13. Hofmann M, et al. 2012. Leukemia. 26:1288. PubMed
  14. Kohn L, et al. 2014. J Immunol. 192:5050. PubMed
  15. Isobe T, et al. 2022. Nat Commun. 13:4501. PubMed
  16. Bigley V, et al. 2015. J Leukoc Biol. 97:627. PubMed
  17. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  18. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  19. Berent-Maoz B, et al. 2015. PLoS One. 10:125414. PubMed
  20. Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
RRID
AB_1877169 (BioLegend Cat. No. 343513) AB_1877168 (BioLegend Cat. No. 343514)

Antigen Details

Structure
105-120 kD single chain mucin-like glycoprotein
Distribution

Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts

Function
Cell adhesion
Ligand/Receptor
L-selectin, E-selectin
Cell Type
Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Krause DS, et al. 1996. Blood 87:1.
2. Puri KD, et al. 1995. J. Cell Biol. 131:261.
3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey.

Gene ID
947 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org

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