Alexa Fluor® 700 anti-human CD34 Antibody
- 产品名称：
- Alexa Fluor® 700 anti-human CD34 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 343525
- 产品应用：
- 343525
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1488 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- Cynomolgus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
The 581 antibody recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press.
- Felschow DM, et al. 2001. Blood 97:3768.
- Rudin CE, et al. 1997. Br. J. Haematol. 97:488.
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC)
- Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF)
- Product Citations
-
- Cirovic B, et al. 2020. Cell Host & Microbe. 28(2):322-334. PubMed
- Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed
- Kenswil KJG, et al. 2021. Cell Stem Cell. 28(4):653-670.e11. PubMed
- de Almeida MJ et al. 2017. Cell stem cell. 21(6):725-729 . PubMed
- Dos Santos JC, et al. 2019. Cell Rep. 28:2659. PubMed
- Hegewisch-Solloa E, et al. 2021. J Immunol. 207:950. PubMed
- Lee J, et al. 2015. J Exp Med. 212:385. PubMed
- Breton G, et al. 2015. J Exp Med. 212:401. PubMed
- RRID
- AB_2561494 (BioLegend Cat. No. 343525) AB_2561495 (BioLegend Cat. No. 343526)
- Structure
- 105-120 kD single chain mucin-like glycoprotein
- Distribution
-
Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts
- Function
- Cell adhesion
- Ligand/Receptor
- L-selectin, E-selectin
- Cell Type
- Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Krause DS, et al. 1996. Blood 87:1.
2. Puri KD, et al. 1995. J. Cell Biol. 131:261.
3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey.
- Gene ID
- 947 View all products for this Gene ID
- UniProt
- View information about CD34 on UniProt.org
