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Brilliant Violet 421™ anti-human CD34 Antibo
产品名称:
Brilliant Violet 421™ anti-human CD34 Antibo
产品类别:
抗体
产品编号:
343609
产品应用:
343609
[价格]
规格 价格 库存
25tests ¥ 2448 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The 561 antibody recognizes a class III group?epitope, which is resistant to sialidase/glycolyprotease and chymopapain treatment.

Application References

(PubMed link indicates BioLegend citation)
  1. Croockewit AJ, et al. 1998. Scand. J. Immunol. 47:82.
  2. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
Product Citations
  1. Rosendahl Huber A, et al. 2022. STAR Protoc. 3:101361. PubMed
  2. Brandsma AM, et al. 2021. Blood Cancer Discov. 2:484. PubMed
  3. Yuzuriha A, et al. 2021. Methods Mol Biol. 2454:411. PubMed
  4. de Kanter JK, et al. 2021. Cell Stem Cell. 28:1726. PubMed
  5. Brandsma AM, et al. 2021. Cancer Discov. . PubMed
  6. Vasu S, et al. 2016. Blood. 127: 2879 - 2889. PubMed
  7. Tong J, et al. 2021. Cell Stem Cell. 28(3):502-513.e6. PubMed
  8. Lu YC, et al. 2018. Cell Rep. 25:2083. PubMed
  9. Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed
  10. Evans WS, et al. 2020. Exp Physiol. 105:1408. PubMed
  11. Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed
  12. Nafria M, et al. 2020. Cell Rep. 31:107691. PubMed
  13. Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
  14. Rabold K, et al. 2022. Nat Commun. 13:6149. PubMed
  15. Schielke L, et al. 2022. Front Immunol. 13:916701. PubMed
  16. Nafria M, et al. 2020. STAR Protoc. 1:100130. PubMed
  17. Hasaart KAL, et al. 2022. iScience. 25:103736. PubMed
  18. Seo GY et al. 2018. Cell host & microbe. 24(2):249-260 . PubMed
  19. Ainsworth RI, et al. 2022. Nat Commun. 13:6221. PubMed
  20. Ward D, et al. 2016. Haematologica. 101: 286 - 296. PubMed
  21. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  22. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  23. Crippa S, et al. 2019. J Clin Invest. 129:1566. PubMed
RRID
AB_11147951 (BioLegend Cat. No. 343609) AB_2561358 (BioLegend Cat. No. 343610)

Antigen Details

Structure
105-120 kD single chain mucin-like glycoprotein
Distribution

Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts

Function
Cell adhesion
Ligand/Receptor
L-selecin, E-selectin
Cell Type
Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
CD Molecules
Gene ID
947 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org

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