Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Flow Cytometry^3,4: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 113003/113004)or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)).
ELISA Capture²: The 55R-286 antibody is useful as a capture antibody when paired with the biotinylated 55R-170 antibody (Cat. No. 112904) as the detecting antibody.
Additional reported applications (for the relevant formats) include: Western blotting and immunoprecipitation¹.

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2. Sheehan, K.C., et al. 1995. J. Exp. Med. 181:607. (ELISA Capture)
3. Schlaepfer, D.D., et al. 2007. J. Biol. Chem. 282:17450. (FC)
4. Bell, J.H., et al. 2007. J. Leukocyte Biol. 82:173. (FC)
5. Kuroishi, T., et al. 2008. J. Leukocyte Biol. 83:912.
6. Wang K, et al. 2012. PLoS One. 7:e52924. PubMed
7. Pereira IR, et al. 2015. PLoS Negl Trop Dis. 9:3659. PubMed

1. Pereira I, et al. 2015. PLoS Negl Trop Dis. 9:3659. PubMed
2. Vilar-Pereira G, et al. 2016. Antimicrob Agents Chemother. 60: 4297 - 4309. PubMed
3. Pobezinskaya EL et al. 2019. Front Immunol. 1.079861111 . PubMed
4. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
5. Li H, et al. 2018. Nat Commun. 9:2830. PubMed
6. Habtetsion T et al. 2018. Cell metabolism. 28(2):228-242 . PubMed
7. Wang K, et al. 2012. PLoS One. 7:e52924. PubMed
8. Puimège L, et al. 2015. EMBO Mol Med. 7: 1004-1017. PubMed
9. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
10. Akhtar MN, et al. 2020. PLoS Pathog. 16:e1009136. PubMed

Variety of cell types at low levels

1. Aggarwal, B. B., et al. 1985. Nature 318 665.
2. Chan, F. K.M., et al. 2000. Science 288:2351.
3. Loetscher, H., et al. 1990. Cell 61:351.
4. Rothe, J., et al. 1993. Nature 364:798.