Ultra-LEAF™ Purified anti-mouse CD120a (TNF -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3240	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

E. coli-expressed extracellular domain of the mouse TNFRI protein

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
Block, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Flow Cytometry: For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112904) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).
ELISA Detection¹: The biotinylated 55R-170 antibody is useful as a detection antibody when paired with the Purified 55R-286 antibody (Cat. No. 113002) as the capture antibody.
Additional reported applications (for the relevant formats) include:immunoprecipitation¹, and In vitro and In vivo receptor blocking¹. The Ultra-LEAF?Purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 112905 & 112906).

Application References (PubMed link indicates BioLegend citation)

Sheehan, K. C., et al. 1995. J. Exp. Med. 181:607.

RRID

AB_2819825 (BioLegend Cat. No. 112905) AB_2819826 (BioLegend Cat. No. 112906)

Antigen Details

Structure: TNFR superfamily, 55 kD
Distribution: Variety of cell types at low levels
Function: Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
Ligand/Receptor: TNF-α, LT-α (TNF-β)
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Aggarwal, B. B., et al. 1985. Nature 318 665.
2. Chan, F. K.M., et al. 2000. Science 288:2351.
3. Loetscher, H., et al. 1990. Cell 61:351.
4. Rothe, J., et al. 1993. Nature 364:798.
Gene ID: 21937 View all products for this Gene ID
UniProt: View information about CD120a on UniProt.org

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