PerCP/Cyanine5.5 anti-mouse Siglec H Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1488	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Siglec-H-Fc recombinant fusion protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated withPerCP/Cyanine5.5 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Blasius AL, et al. 2006. J. Immunol. 177:3260.

Product Citations

Liao Y, et al. 2017. Virol J. 10.1186/s12985-017-0801-x. PubMed
Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed

RRID

AB_10639936 (BioLegend Cat. No. 129613) AB_10643995 (BioLegend Cat. No. 129614)

Antigen Details

Structure: Sialic acid-binding immunoglobulin-like lectins
Distribution: Siglec-H is expressed exclusively on natural interferon-producing cells (IPCs), also called plasmacytoid dendritic cells in spleen, blood and lymph nodes. The IPCs are characterized by a complex phenotype (CD11c+, Ly-6C+, B220+, CD11b-, CD8+/-).
Function: Siglec-H associates with adaptor protein DAP12 for cell signaling, which reduces type 1 IFN response of IPC to CpG.
Ligand/Receptor: Sialic acid
Cell Type: Dendritic cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Siglec Molecules
Antigen References: 1. Blasius AL, et al. 2006. Blood 107:2474.
2. Zhang J, et al. 2006. Blood 107:3600.
3. Blasius AL, et al. 2007. J. Exp. Med. 204:2561.
Gene ID: 233274 View all products for this Gene ID
UniProt: View information about Siglec H on UniProt.org

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