Alexa Fluor® 488 anti-human HIF1α Antibo
- 产品名称：
- Alexa Fluor® 488 anti-human HIF1α Antibo
- 产品类别：
- 抗体
- 产品编号：
- 359707
- 产品应用：
- 359707
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2052 |
1 |
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant HIF1α
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Product Citations
-
- Kotzé LA, et al. 2021. mSphere. 6:e0055221. PubMed
- Martínez-Fábregas J, et al. 2020. Cell Rep. 33:108545. PubMed
- RRID
- AB_2563975 (BioLegend Cat. No. 359707) AB_2563976 (BioLegend Cat. No. 359708)
- Structure
- 92.6 kD, contains one basic helix-loop-helix structure (bHLH), one PAS associated C-terminal (PAC) domain and two PER-ARNT-SIM (PAS) domains.
- Distribution
- Under normoxic conditions, detected in the cytoplasm; oxygen starvation causes nuclear translocation.
- Function
- Master regulator of hypoxia in the adaptive immune response; under low oxygen conditions, induces expression of multiple genes, such as VEGF, erythropoietin, and other genes that aid in adaptation to low oxygen concentrations.
- Interaction
- Heterodimer binds 5'-[AG]CGTG-3' sequence of DNA in the hypoxia response element of target genes; interacts with COPS5, EP300, CITED2.
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Wang GL and Semenza GL.?1993. Proc. Natl. Acad. Sci. 90:4304.
2. Wang GL and Semenza GL.?1993. J. Biol. Chem. 268:21513.
3. Wang GL and Semenza GL.?1995. J. Biol. Chem. 270:1230.
4. Galbraith MD, et al. 2013. Cell. 153:1327.
- Gene ID
- 3091 View all products for this Gene ID
- UniProt
- View information about HIF1a on UniProt.org
