PerCP/Cyanine5.5 anti-mouse CD5 Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse CD5 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 100623
- 产品应用：
- 100623
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse thymus or spleen
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
- Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC)
- Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370.
- Product Citations
-
- Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
- Català C, et al. 2022. iScience. 25:105078. PubMed
- Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
- Kobayashi T, et al. 2019. Cell. 176:982. PubMed
- Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
- Snyder LM, et al. 2022. Immunohorizons. 6:660. PubMed
- Bennion BG, et al. 2020. Cell Reports. 31(11):107771. PubMed
- Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
- Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed
- McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
- Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
- Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
- Nasarre P, et al. 2021. Cancers (Basel). 13: . PubMed
- Peng V, et al. 2022. Nat Immunol. 23:619. PubMed
- Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
- Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
- Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
- Borrelli MR, et al. 2020. Stem Cells Transl Med. 1.347916667. PubMed
- Goc J, et al. 2021. Cell. :. PubMed
- RRID
- AB_2563432 (BioLegend Cat. No. 100623) AB_2563433 (BioLegend Cat. No. 100624)
- Structure
- Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD
- Distribution
-
Thymocytes, T cells, B-1 cells
- Function
- Negative regulator of T-B cell interaction
- Ligand/Receptor
- CD72, gp35-37
- Cell Type
- B cells, T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kipps TJ. 1988. Adv. Immunol. 47:117.
3. Antin JH, et al. 1985. J. Immunol. 136:505.
4. Tarakhovsky A, et al. 1995. Science 269:535.
- Gene ID
- 12507 View all products for this Gene ID
- UniProt
- View information about CD5 on UniProt.org
