PerCP anti-mouse CD5 Antibody
- 产品名称:
- PerCP anti-mouse CD5 Antibody
- 产品类别:
- 抗体
- 产品编号:
- 100615
- 产品应用:
- 100615
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse thymus or spleen
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.
- Application References
(PubMed link indicates BioLegend citation) -
- Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
- Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC)
- Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370.
- Product Citations
-
- Plzakova L, et al. 2014. Microb Pathog. 75:49. PubMed
- Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed
- RRID
- AB_2260093 (BioLegend Cat. No. 100615) AB_2075300 (BioLegend Cat. No. 100616)
- Structure
- Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD
- Distribution
-
Thymocytes, T cells, B-1 cells
- Function
- Negative regulator of T-B cell interaction
- Ligand/Receptor
- CD72, gp35-37
- Cell Type
- B cells, T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kipps TJ. 1988. Adv. Immunol. 47:117.
3. Antin JH, et al. 1985. J. Immunol. 136:505.
4. Tarakhovsky A, et al. 1995. Science 269:535.
- Gene ID
- 12507 View all products for this Gene ID
- UniProt
- View information about CD5 on UniProt.org