PE anti-mouse CD5 Antibody
- 产品名称：
- PE anti-mouse CD5 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 100607
- 产品应用：
- 100607
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse thymus or spleen
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.
- Application References
(PubMed link indicates BioLegend citation) -
- Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
- Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC)
- Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370.
- Product Citations
-
- Liu J, et al. 2021. Front Immunol. 12:747730. PubMed
- Barrow AD et al. 2018. Cell. 172(3):534-548 . PubMed
- LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
- Solanki A, et al. 2018. Development. 145. PubMed
- Webster P, et al. 2018. Nat Commun. 9:2649. PubMed
- Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
- Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
- LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
- Kang X, et al. 2022. J Immunol Res. 2022:8118577. PubMed
- Xu M, et al. 2013. J Immunol. 190:5436. PubMed
- Orlanski S, et al. 2016. Proc Natl Acad Sci U S A. 113: 5018-5023. PubMed
- Xu C, et al. 2020. Immunity. 53(2):417-428.e4. PubMed
- Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
- Coley WD et al. 2018. F1000Research. 2018 Mar 14 [revised 2018 Jan 1] 0.5125. PubMed
- Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
- Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
- Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
- Evavold CL, et al. 2021. Cell. 184(17):4495-4511.e19. PubMed
- Hirose T, et al. 2017. PLoS One. 12(6):e0178765. PubMed
- Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
- Snapper Q 2013. J Immunol. 190:1048. PubMed
- RRID
- AB_312736 (BioLegend Cat. No. 100607) AB_312737 (BioLegend Cat. No. 100608)
- Structure
- Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD
- Distribution
-
Thymocytes, T cells, B-1 cells
- Function
- Negative regulator of T-B cell interaction
- Ligand/Receptor
- CD72, gp35-37
- Cell Type
- B cells, T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kipps TJ. 1988. Adv. Immunol. 47:117.
3. Antin JH, et al. 1985. J. Immunol. 136:505.
4. Tarakhovsky A, et al. 1995. Science 269:535.
- Gene ID
- 12507 View all products for this Gene ID
- UniProt
- View information about CD5 on UniProt.org
