Pacific Blue™ anti-mouse CD5 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1692	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse thymus or spleen

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Pacific Blue? under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, and immunohistochemistry² of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.

Application References (PubMed link indicates BioLegend citation)

Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC)
Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370.

RRID

AB_2813915 (BioLegend Cat. No. 100641) AB_2813916 (BioLegend Cat. No. 100642)

Antigen Details

Structure: Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD
Distribution: Thymocytes, T cells, B-1 cells
Function: Negative regulator of T-B cell interaction
Ligand/Receptor: CD72, gp35-37
Cell Type: B cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kipps TJ. 1988. Adv. Immunol. 47:117.
3. Antin JH, et al. 1985. J. Immunol. 136:505.
4. Tarakhovsky A, et al. 1995. Science 269:535.
Gene ID: 12507 View all products for this Gene ID
UniProt: View information about CD5 on UniProt.org

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