APC anti-mouse CD5 Antibody
- 产品名称：
- APC anti-mouse CD5 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 100625
- 产品应用：
- 100625
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse thymus or spleen
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.
- Application References
(PubMed link indicates BioLegend citation) -
- Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
- Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC)
- Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370.
- Product Citations
-
- Kawabe T, et al. 2020. Nat Commun. 2.795833333. PubMed
- Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed
- Jennings EK, et al. 2021. STAR Protocols. 2(1):100284. PubMed
- Han J, et al. 2018. BMC Res Notes. 11:229. PubMed
- Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed
- Hojo MA, et al. 2019. Nat Commun. 2.224305556. PubMed
- Xu H, et al. 2020. Immunity. 51(4):696-708.e9.. PubMed
- Kim S, et al. 2021. Elife. 10:. PubMed
- Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
- Zhang Q, et al. 2021. Front Cell Dev Biol. 9:655552. PubMed
- Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed
- Dong X, et al. 2022. Front Immunol. 13:896472. PubMed
- Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
- Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
- Werner A, et al. 2021. iScience. 24:103076. PubMed
- Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
- Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
- Goc J, et al. 2021. Cell. :. PubMed
- Liang Z, et al. 2022. iScience. 25:105233. PubMed
- Whiteley AM et al. 2017. eLife. 6 pii: e26435. PubMed
- RRID
- AB_2563928 (BioLegend Cat. No. 100625) AB_2563929 (BioLegend Cat. No. 100626)
- Structure
- Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD
- Distribution
-
Thymocytes, T cells, B-1 cells
- Function
- Negative regulator of T-B cell interaction
- Ligand/Receptor
- CD72, gp35-37
- Cell Type
- B cells, T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kipps TJ. 1988. Adv. Immunol. 47:117.
3. Antin JH, et al. 1985. J. Immunol. 136:505.
4. Tarakhovsky A, et al. 1995. Science 269:535.
- Gene ID
- 12507 View all products for this Gene ID
- UniProt
- View information about CD5 on UniProt.org
