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首页 > 抗体蛋白 > 抗体
Pacific Blue™ anti-mouse CD8a Antibody
产品名称：
Pacific Blue™ anti-mouse CD8a Antibody
产品类别：
抗体
产品编号：
100728
产品应用：
100728
[价格]
规格 价格 库存
25µg ￥ 1128 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD8a antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3, in vivo and in vitro cell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP)
  2. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  3. Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP)
  4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Kamimura D, et al. 2006. J. Immunol. 177:306.
  9. Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete)
  10. Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed
  11. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  12. Rasmussen JW, et al. 2006. Infect. Immun. 74:6590. PubMed
  13. Lee CH, et al. 2009. Clin. Cancer Res. PubMed
  14. Geiben-Lynn R, et al. 2008. Blood 112:4585. (Deplete) PubMed
  15. Kingeter LM, et al. 2008. J. Immunol. 181:6244. PubMed
  16. Guo Y, et al. 2008. Blood 112:480. PubMed
  17. Andrews DM, et al. 2008. J. Virol. 82:4931. PubMed
  18. Britschqui MR, et al. 2008. J. Immunol. 181:7681. PubMed
  19. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  20. Jordan JM, et al. 2008. Infect. Immun. 76:3717. PubMed
  21. Todd DJ, et al. 2009. J. Exp. Med. 206:2151. PubMed
  22. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  23. Medyouf H, et al. 2010. Blood 115:1175. PubMed
  24. Riedl P, et al. 2009. J. Immunol. 183:370. PubMed
  25. Apte SH, et al. 2010. J. Immunol. 185:998. PubMed
  26. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  27. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  28. Cui L, et al. 2015. J Control Release. 206:220. PubMed
  29. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  30. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Tan L, et al. 2022. Aging Dis. 13:1562. PubMed
  2. Chen Y, et al. 2020. Virol Sin. . PubMed
  3. Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed
  4. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  5. Gawish R, et al. 2022. Elife. 11:. PubMed
  6. Zhu W, et al. 2021. Onco Targets Ther. 14:2149. PubMed
  7. Fan L, et al. 2021. Cancers (Basel). 13:. PubMed
  8. Hamilton JAG, et al. 2021. Aging Cell. 20:e13309. PubMed
  9. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
  10. Rosenkranz SC, et al. 2021. eLife. 10:00. PubMed
  11. Rezende R, et al. 2015. Nat Commun. 6: 8726. PubMed
  12. Schneider C, et al. 2018. Cell. 174:271. PubMed
  13. Page N, et al. 2018. Immunity. 48:937. PubMed
  14. Wabitsch S, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1166. PubMed
  15. Engler J, et al. 2017. Proc Natl Acad Sci U S A. 114(2):E181-E190. PubMed
  16. Gupta SS, et al. 2019. Cell Rep. 29:1862. PubMed
  17. Gu M, et al. 2021. Nat Immunol. 22:193. PubMed
  18. Galeano Ni?o JL, et al. 2020. J Cell Sci. . PubMed
  19. Saito S, et al. 2020. Nutrients. 12:. PubMed
  20. Wabitsch S, et al. 2021. STAR Protoc. 2:100517. PubMed
  21. Molofsky A, et al. 2013. J Exp Med. 210:535. PubMed
  22. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  23. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
  24. Liang Z, et al. 2021. J Cell Physiol. 236:4725. PubMed
  25. Price J, et al. 2015. Diabetes. 64: 3521 - 3531. PubMed
  26. Sato Y, et al. 2021. J Clin Invest. Online ahead of print. PubMed
  27. Ontiveros-Padilla L, et al. 2021. Microorganisms. 9:. PubMed
  28. Li H, et al. 2022. Theranostics. 12:6422. PubMed
  29. Geiger R, et al. 2016. Cell. 167:829-842. PubMed
  30. Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed
  31. Sokol CL et al. 2018. Immunity. 49(3):449-463 . PubMed
  32. Terashima Y, et al. 2020. Nat Commun. 11:609. PubMed
  33. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  34. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  35. Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed
  36. Atkinson S, et al. 2016. Dis Model Mech. 9: 427-440. PubMed
  37. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  38. Jasti S, et al. 2017. Biol Reprod. 96:134. PubMed
  39. Kulkarni R, et al. 2021. PLoS One. 16:e0257191. PubMed
  40. Melissa M Remy et al. 2017. Cell host & microbe. 22(3):354-365 . PubMed
  41. Galeano Ni?o JL, et al. 2020. J Cell Sci. 133:00:00. PubMed
  42. Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
  43. Van Braeckel-Budimir N, et al. 2018. Cell Rep. 24:3374. PubMed
  44. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  45. De Ponte Conti B, et al. 2021. Elife. 10:. PubMed
  46. Chun N, et al. 2021. JCI Insight. 6:. PubMed
  47. Daneshmandi S, et al. 2021. Elife. 10:. PubMed
  48. Tian Y, et al. 2016. J Immunol. 197: 1308 - 1321. PubMed
  49. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  50. Ren Y, et al. 2015. Sci Rep. 5: 18099. PubMed
  51. K?lin S et al. 2017. Cell metabolism. 26(3):475-492 . PubMed
  52. López MJ, et al. 2018. Invest Ophthalmol Vis Sci. 59:5671. PubMed
  53. Zhang Q, et al. 2021. Front Cell Dev Biol. 9:655552. PubMed
  54. Blanchard L, et al. 2022. STAR Protoc. 3:101444. PubMed
  55. Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  56. Mohebiany AN, et al. 2020. Cell Reports. 30(5):1585-1597.e6.. PubMed
  57. Tian Q, et al. 2022. Front Mol Neurosci. 15:850904. PubMed
  58. Kwok T, et al. 2022. Front Aging. 3:838943. PubMed
  59. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  60. Engblom C, et al. 2017. Science. 358:6367. PubMed
  61. Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
  62. O'Leary CE, et al. 2021. Curr Protoc. 1:e77. PubMed
  63. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
  64. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
  65. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  66. Yu H, et al. 2021. Cell Death Dis. 13:1. PubMed
  67. Zhang Z, et al. 2020. Nature. 579:415. PubMed
  68. Wang X, et al. 2022. Elife. 11:. PubMed
  69. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  70. Akhand SS, et al. 2020. Cancer Immunol Res. 8:1542. PubMed
  71. Chen D, et al. 2021. JCI Insight. 6:. PubMed
  72. Gawish R, et al. 2022. Elife. 11:. PubMed
  73. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  74. Pflügler S, et al. 2020. Commun Biol. 3:252. PubMed
  75. Quijano-Rubio A, et al. 2022. Nat Biotechnol. Online ahead of print. PubMed
  76. Wolf Y, et al. 2019. Cell. 179:219. PubMed
RRID
AB_493426 (BioLegend Cat. No. 100728) AB_493425 (BioLegend Cat. No. 100725)

Antigen Details

Structure
Ig superfamily, CD8α chain, 34 kD
Distribution

Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells

Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.

Gene ID
12525 View all products for this Gene ID
UniProt
View information about CD8a on UniProt.org

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