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Alexa Fluor® 647 anti-mouse CD8a Antibody
产品名称：
Alexa Fluor® 647 anti-mouse CD8a Antibody
产品类别：
抗体
产品编号：
100727
产品应用：
100727
[价格]
规格 价格 库存
25µg ￥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC - Verified

SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L?volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0?μg/mL?is suggested.?For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3, in vivo and in vitro cell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP)
  2. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  3. Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP)
  4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Kamimura D, et al. 2006. J. Immunol. 177:306.
  9. Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete)
  10. Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed
  11. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  12. Rasmussen JW, et al. 2006. Infect. Immun. 74:6590. PubMed
  13. Lee CH, et al. 2009. Clin. Cancer Res. PubMed
  14. Geiben-Lynn R, et al. 2008. Blood 112:4585. (Deplete) PubMed
  15. Kingeter LM, et al. 2008. J. Immunol. 181:6244. PubMed
  16. Guo Y, et al. 2008. Blood 112:480. PubMed
  17. Andrews DM, et al. 2008. J. Virol. 82:4931. PubMed
  18. Britschqui MR, et al. 2008. J. Immunol. 181:7681. PubMed
  19. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  20. Jordan JM, et al. 2008. Infect. Immun. 76:3717. PubMed
  21. Todd DJ, et al. 2009. J. Exp. Med. 206:2151. PubMed
  22. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  23. Medyouf H, et al. 2010. Blood 115:1175. PubMed
  24. Riedl P, et al. 2009. J. Immunol. 183:370. PubMed
  25. Apte SH, et al. 2010. J. Immunol. 185:998. PubMed
  26. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  27. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  28. Cui L, et al. 2015. J Control Release. 206:220. PubMed
  29. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  30. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Minute L, et al. 2020. J Immunother Cancer. 8:. PubMed
  2. Djokić V, et al. 2019. Front Immunol. 9:2891. PubMed
  3. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  4. Parlane N, et al. 2013. Vet Immunol Immunopathol. 30:122. PubMed
  5. Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed
  6. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  7. Park JJ, et al. 2021. Nat Commun. 12:1222. PubMed
  8. Kim K, et al. 2009. PLoS One. 4:e7738. PubMed
  9. Tang B, et al. 2020. Clin Cancer Res. 26:2216. PubMed
  10. Peng Z, et al. 2021. STAR Protocols. 2(2):100595. PubMed
  11. Najjar YG, et al. 2019. JCI Insight. 4:. PubMed
  12. Dai B, et al. 2022. Theranostics. 12:7603. PubMed
  13. Ma YV, et al. 2021. MAbs. 13:2003281. PubMed
  14. Etxeberria I, et al. 2020. Cancer Cell. 36(6):613-629. PubMed
  15. Schuhmann MK, et al. 2021. J Neuroinflammation. 18:46. PubMed
  16. Seedhom M, et al. 2016. J Immunol. 197: 1498 - 1506. PubMed
  17. Young R, et al. 2015. Proc Natl Acad Sci U S A. 112: 13447 - 13454. PubMed
  18. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
  19. Kim SH, et al. 2021. Cell Reports. 35(2):108995. PubMed
  20. Wang F, et al. 2019. MAbs. 12:1685350. PubMed
  21. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  22. Britschgi M, et al. 2008. J Immunol. 181:7681. PubMed
  23. Koikawa K, et al. 2021. Cell. 184(18):4753-4771.e27. PubMed
  24. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  25. Matryba P, et al. 2020. J Immunol. 1395:204. PubMed
  26. Yu M, et al. 2021. Molecular Cell. 81(6):1216-1230.e9. PubMed
  27. Tanaka Y, et al. 2017. J Immunol. 199:4016. PubMed
  28. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
  29. Zhang Y, et al. 2021. Commun Biol. 344:4. PubMed
  30. Werner A, et al. 2021. iScience. 24:103076. PubMed
  31. Rui J, et al. 2021. Nat Commun. 12:5074. PubMed
  32. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  33. Xiao M, et al. 2022. Mol Oncol. 16:1026. PubMed
  34. Yin Y, et al. 2016. J Biol Chem. 291: 6923 - 6935. PubMed
  35. Zhou Y, et al. 2016. Sci Rep. 6:28140. PubMed
  36. Richardson M, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  37. Rasmussen J, et al. 2006. Infect Immun. 74:6590. PubMed
RRID
AB_493424 (BioLegend Cat. No. 100727) AB_389326 (BioLegend Cat. No. 100724)

Antigen Details

Structure
Ig superfamily, CD8α chain, 34 kD
Distribution

Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells

Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.

Gene ID
12525 View all products for this Gene ID
UniProt
View information about CD8a on UniProt.org

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