Pacific Blue™ anti-mouse CD8b.2 Antibody
- 产品名称：
- Pacific Blue™ anti-mouse CD8b.2 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 140414
- 产品应用：
- 140414
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse thymus or spleen
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence3, immunohistochemical staining4 of frozen tissue section using dry ice-isopentane method and immunoprecipitation5.
- Application References
(PubMed link indicates BioLegend citation) -
- Ledbetter J, et al. 1979. Immunol. Rev. 47:63.
- Ledbetter J, et al. 1980. J. Exp. Med. 152:280.
- Vremec D, et al. 2000. J. Immunol. 164:2978. (IF)
- Lawrence D, et al. 1999. J. Virol. 73:1795. (IHC)
- Bosselut R, et al. 1999. J. Exp. Med. 190:1517. (IP)
- Product Citations
-
- Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed
- Lercher A, et al. 2019. Immunity. 51:1074. PubMed
- Nika L, et al. 2019. Vaccines (Basel). 7. PubMed
- Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
- Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
- RRID
- AB_10641278 (BioLegend Cat. No. 140414)
- Structure
- Ig superfamily, CD8β chain, 32 kD
- Distribution
-
Majority of thymocytes, cytotoxic T cell subsets
- Function
- Co-receptor of TCR
- Ligand/Receptor
- MHC class I
- Cell Type
- T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, TCRs
- Antigen References
-
1. Ledbetter J, et al. 1981. J. Exp. Med. 153:1503.
2. Renard V, et al. 1996. J. Exp. Med. 184:2439.
- Gene ID
- 12526 View all products for this Gene ID
- UniProt
- View information about CD8b.2 on UniProt.org
