APC anti-mouse CD8b.2 Antibody
- 产品名称：
- APC anti-mouse CD8b.2 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 140410
- 产品应用：
- 140410
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse thymus or spleen
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence3, immunohistochemical staining4 of frozen tissue section using dry ice-isopentane method and immunoprecipitation5.
- Application References
(PubMed link indicates BioLegend citation) -
- Ledbetter J, et al. 1979. Immunol. Rev. 47:63.
- Ledbetter J, et al. 1980. J. Exp. Med. 152:280.
- Vremec D, et al. 2000. J. Immunol. 164:2978. (IF)
- Lawrence D, et al. 1999. J. Virol. 73:1795. (IHC)
- Bosselut R, et al. 1999. J. Exp. Med. 190:1517. (IP)
- Product Citations
-
- Montes de Oca R, et al. 2021. Mol Cancer Ther. 20:1941. PubMed
- Guo L, et al. 2021. Front Immunol. 12:785457. PubMed
- Zhou H, et al. 2022. Oncoimmunology. 11:2057892. PubMed
- Michels A, et al. 2021. Mol Ther Methods Clin Dev. 23:334. PubMed
- Evgin L, et al. 2022. Sci Transl Med. 14:eabn2231. PubMed
- Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
- Zhou H, et al. 2021. Cell Death Discov. 7:332. PubMed
- Kurashima Y, et al. 2021. Nat Commun. 12:1067. PubMed
- Mazumder S, et al. 2021. Heliyon. 7:e08124. PubMed
- Louise V Webb et al. 2019. Immunity. 50(2):348-361 . PubMed
- RRID
- AB_10641696 (BioLegend Cat. No. 140410)
- Structure
- Ig superfamily, CD8β chain, 32 kD
- Distribution
-
Majority of thymocytes, cytotoxic T cell subsets
- Function
- Co-receptor of TCR
- Ligand/Receptor
- MHC class I
- Cell Type
- T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, TCRs
- Antigen References
-
1. Ledbetter J, et al. 1981. J. Exp. Med. 153:1503.
2. Renard V, et al. 1996. J. Exp. Med. 184:2439.
- Gene ID
- 12526 View all products for this Gene ID
- UniProt
- View information about CD8b.2 on UniProt.org
